#course/2005nsc

3.1 - Protein diversity & techniques to study proteins

Introduction To fully understand how a protein's structure can be identified, we must first understand it's physical and chemical properties. Basically a protein simply has 5 properties 3 physical and 2 chemical.…

3.2 - Dialysis

Before being able to analyze the structure of a protein, we must be able to purify a sample of proteins. Dialysis is a method of purifying a protein sample by removing small…

3.3 - Techniques and Reagents

Once we understand the properties of a protein, we can identify multiple different ways to sort proteins to figure out its composition. Protein Analysis Techniques SDS PAGE Principle: Separates proteins by size…

3.4 N-Terminal amino Acid Sequencing

5. N terminal Amino Acid Sequencing Purpose: Identify the sequence of amino acids at the protein’s N terminus. Techniques: Edman Degradation: Sequential removal of N terminal residues. Sensitivity: Detects down to fmole…